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Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
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Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
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Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
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Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
Human Embryonic Lung Fibroblasts (Helf, supplied by Biolot, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+embryonic+lung+fibroblasts+helf/human+embryonic+lung+fibroblasts++helf+/pmc10417023-89-0-13
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ATCC human embryonic lung fibroblast helf
Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
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human embryonic lung fibroblasts helf  (ATCC)
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ATCC human embryonic lung fibroblasts helf
Embryonic lung <t>fibroblasts</t> stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates
Human Embryonic Lung Fibroblasts Helf, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+embryonic+lung+fibroblasts+helf/Primary+Lung+Fibroblast%3B+Fibrosis/pm36463525-34-14-22
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human embryonic lung fibroblasts helf - by Bioz Stars, 2026-10
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Embryonic lung fibroblasts stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates

Journal: Stem Cell Research & Therapy

Article Title: Influence of mesenchymal and biophysical components on distal lung organoid differentiation

doi: 10.1186/s13287-024-03890-2

Figure Lengend Snippet: Embryonic lung fibroblasts stimulate the differentiation of lung progenitors into HT-280 + AEC2 cells. A Top panel: Schematic of the indirect Transwell® co-culture system to determine the effect of irradiated human embryonic lung fibroblasts (ihELF) on the differentiation of D22 NKX2-1 + LPCs into AEC2. Bottom panel: Flow cytometry analysis for CDH1 and HT2-280 of organoids grown in different matrices. Data are expressed as mean ± SEM, n = 4 biological replicates. Dashed lines indicate mean % of HT2-280 positive cells, respectively, after combining the matrix data in both groups. * p < 0.05 by Mann–Whitney U test, no ihELF versus ihELF. B Left panel: Schematic of experimental design to determine the effect of direct versus indirect contact of LPCs with ihELF on the differentiation of D22 NKX2-1 + LPCs to AEC2. Right panel: Flow cytometry analysis for HT2-280 of organoids grown directly versus indirectly with ihELF after 20 days of culture. Data are expressed as mean ± SEM, n ≥ 10 biological replicates

Article Snippet: Human embryonic lung fibroblasts (hELF, 17.5 weeks of gestation, DV Biologics) were cultured in DMEM (Gibco, #11965118) containing 10% (v/v) FBS and 1% (v/v) penicillin/streptomycin.

Techniques: Co-Culture Assay, Irradiation, Flow Cytometry, MANN-WHITNEY